Plasmid

Part:BBa_K4188004:Design

Designed by: Tobias Bröker   Group: iGEM22_WWU_Muenster   (2022-09-28)


pSB3CY_Trp shuttle vector with tryptophane synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6802
    Illegal XbaI site found at 3425
    Illegal SpeI site found at 3790
    Illegal PstI site found at 1105
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6802
    Illegal NheI site found at 5226
    Illegal NheI site found at 5488
    Illegal SpeI site found at 3790
    Illegal PstI site found at 1105
    Illegal NotI site found at 5429
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6802
    Illegal BamHI site found at 2441
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6802
    Illegal XbaI site found at 3425
    Illegal SpeI site found at 3790
    Illegal PstI site found at 1105
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6802
    Illegal XbaI site found at 3425
    Illegal SpeI site found at 3790
    Illegal PstI site found at 1105
    Illegal AgeI site found at 793
    Illegal AgeI site found at 905
    Illegal AgeI site found at 2083
    Illegal AgeI site found at 2406
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1378
    Illegal BsaI site found at 6808
    Illegal BsaI.rc site found at 1099
    Illegal SapI site found at 1082
    Illegal SapI.rc site found at 6


Design Notes

For more information about the design of this vector, you can visit the page BBa_K4188001.

Source

The auxotrophy has already been added to the ready-to-use shuttle vectors to make their use as easy and as fast as possible. For more information on the origin, plasmid visit the part BBa_K4188001.

References